Molecular Phylogenetics of Coastal Invertebrates
An investigation into the evolutionary relationships and population structure of intertidal invertebrate communities, combining morphological taxonomy with molecular sequence data (COI, 16S) to resolve invertebrate species boundaries. Maximum likelihood and Bayesian inference analyses were used to construct well-supported phylogenies, revealing previously unrecognised lineages within two focal genera.
Methods
- DNA extraction & PCR amplification
- Sequence alignment (MUSCLE)
- ML inference (IQ-TREE)
- Bayesian inference (MrBayes)
- Species delimitation (ABGD, GMYC)